OkuhlosiweSebenzisa
Lo mkhiqizo ufanelekele ukutholwa kwekhwalithi ye-COVID-19.Inikeza usizo ekuxilongweni kokutheleleka nge-coronavirus yenoveli.
ISIFINYEZO
Ama-coronavirus anoveli awohlobo lwe-β.I-COVID-19 yisifo esithathelwanayo sokuphefumula esinamandla.Ngokuvamile abantu bayathinteka.Njengamanje, iziguli ezingenwe yi-coronavirus yenoveli ziwumthombo oyinhloko wokutheleleka;abantu abane-asymptomatic abangenwe yileli gciwane bangaba umthombo othathelwanayo.Ngokusekelwe ophenyweni lwamanje lwe-epidemiological, isikhathi sokufukamela siyizinsuku ezi-1 kuya kweziyi-14, izikhathi eziningi izinsuku ezi-3 kuya kweziyi-7.Izimpawu eziyinhloko zihlanganisa umkhuhlane, ukukhathala nokukhwehlela okomile.Ukucinana kwamakhala, ikhala eligijimayo, umphimbo obuhlungu, i-myalgia kanye nesifo sohudo kutholakala ezimweni ezimbalwa.
ISIMISO
Ikhithi yokuhlola iqukethe imicu yokuhlola emibili:
Kwenye yazo, i-burgundy colored conjugate pad equkethe i-Novel coronavirus recombinant envelope antigens ehlanganiswe ne-Colloid gold (Novel coronavirus conjugates), 2) umugqa we-nitrocellulose oqukethe imigqa yokuhlola emibili (imigqa ye-IgG ne-IgM) kanye nolayini wokulawula (umugqa we-C1) .
Ulayini we-IgM umbozwe ngaphambili nge-antibody IgM yegundane, ulayini we-IgG umbozwe nge-Mouse anti-Human IgG antibody.Uma ivolumu eyanele yesampula yokuhlola ikhishelwa kusampula yomthombo wedivayisi yokuhlola, isifanekiso sihamba ngesenzo se-capillary kulo lonke idivayisi.I-IgM anti-Novel coronavirus, uma ikhona kusampula, izobophezela ku-conjugates ye-Novel coronavirus.
I-immunocomplex ibe isithathwa yi-reagent embozwe ngaphambili ebhendini ye-IgM, yakhe umugqa we-IgM onombala obomvana, okhombisa umphumela wokuhlola we-Novel coronavirus IgM.I-IgG anti-Novel coronavirus uma ikhona kusampula izobophezela kuma-conjugates e-Novel coronavirus.I-immunocomplex ibe isithathwa yi-reagent ehlanganiswe kulayini we-IgG, yakha umugqa we-IgG onemibala ebomvana, ekhombisa umphumela wokuhlola we-Novel coronavirus IgG.Ukungabikho kwanoma yimiphi imigqa ye-T (IgG ne-IgM) kuphakamisa umphumela ongemuhle.Ukuze usebenze njengesilawuli senqubo, ulayini onemibala uzohlala uvela endaweni yomugqa wokulawula obonisa ukuthi umthamo ofanele wesifanekiso sengeziwe futhi i-membrane wicking yenzekile.
Komunye umugqa, umugqa wokuhlola wakhiwe izingxenye ezilandelayo: okuyiphedi yesampula, iphedi ye-reagent, i-membrane yokusabela, nephedi yokumunca.Iphedi ye-reagent iqukethe i-colloidal-gold conjugated ne-monoclonal antibodies ngokumelene ne-nucleocapsid protein ye-SARS-CoV-2;i-membrane yokusabela iqukethe amasosha omzimba wesibili we-nucleocapsid protein ye-SARS-CoV-2.Umucu wonke ufakwe ngaphakathi kwensimbi yepulasitiki.Lapho isampula yengezwa emthonjeni wesampula, ama-conjugate omisiwe kuphedi ye-reagent ayahlakazwa futhi afuduke kanye nesampula.Uma i-antigen yakwa-SARS-CoV-2 yethula kusampula, inkimbinkimbi eyakhiwe phakathi kwe-anti-SARS-2 conjugate kanye negciwane izobanjwa ama-anti-SARS-2 monoclonal antibodies ambozwe esifundeni somugqa wokuhlola (T).Ukungabikho komugqa T kuphakamisa umphumela ongemuhle.Ukuze usebenze njengokulawula kwenqubo umugqa obomvu uzohlala uvela esifundeni somugqa wokulawula (C2) okubonisa ukuthi ivolumu efanele yesampula yengeziwe futhi i-membrane wicking yenzekile.
Isikhathi sokuthumela: Apr-01-2021