I-Novel Coronavirus (2019-nCoV) Ikhithi Yokuthola I-Ribonucleic Acid (I-PCR yesikhathi sangempela - I-Fluorescent Probe Assay)
OkuhlosiweSebenzisa
Lo mkhiqizo uhloselwe ukutholwa kwekhwalithi ye-novel coronavirus (SARS-CoV-2) kumasampula okuphefumula.Imiphumela yokuhlonza ingasetshenziswa ukusiza ukuxilonga emtholampilo kwe-Coronavirus Disease 2019 (COVID-19).
Izingxenye
2019-nCoV PCR Buffer - 96 test
I-PCR Enzyme Mix - izivivinyo ezingama-96
2019-nCoV Positive Control - izivivinyo ezingama-48
Ukulawula Okungalungile kwe-2019-nCoV - izivivinyo ezingama-48
Ukulawula Kwangaphakathi kwe-2019-nCoV - ukuhlolwa okungu-96
Faka Iphakheji - ikhophi engu-1
UmkhiqizoIndlela
Lo mkhiqizo uqukethe amapheya amane we-PCR primers, ama-fluorescent probes amane, i-RNA reverse transcriptase, i-DNA polymerase, i-dNTP, i-magnesium ion, namanye amakhemikhali okutholwa kwe-RNA yegciwane.Iqondise ohlotsheni lofuzo lwe-RNA polymerase (RdRp) oluncike ku-RNA, ufuzo lwe-Nucleocapsid (N), kanye nofuzo lwe-Envelop (E) luka-2019-nCoV ngesikhathi esisodwa kushubhu eyodwa yokuhlola.Inqubo yokuhlola ihamba ngezinyathelo ezintathu ezilandelayo: 1) Hlehlisa ukuloba i-RNA yeviral ibe yi-viral DNA.2) Khulisa i-DNA yegciwane ibe yinani elilinganisekayo.3) Bika inani lama-amplicon e-DNA ngokusebenzisa i-probe hybridization.Ngaphandle kwalokho, lo mkhiqizo uhlanganisa nokulawula kwangaphakathi nokulawula kwangaphandle ukuze kuqashwe ukusebenza kwawo ngesikhathi sokuthutha nokugcinwa.